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Both the femur and vertebrae L6 volumes may have seen an increase, but there didnt seem to be a change in the volumetric BMDs

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The initial model was rigid-body fitted into the consensus EM density map via UCSF Chimera 1.14 32 , followed by manual adjustment using ISOLDE 33 embedded in UCSF ChimeraX 1.6 34

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The beads were collected and washed, and the bound proteins were eluted by Laemmli buffer containing 500 L 6 M urea, 25 L 200 mM DTT, and 25 L 500 mM IAA in the dark at 25C for 30 minutes