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To quarantee similar LC conditions for all experiments, column decoppering with10 l of 1 mM EDTA was performed before each LC ICP MS experiment
Materials and methods Cell culture and characterization Human iPSCs (gifted from Bonyakhteh Stem Cell Research Center, Tehran, Iran) were maintained on mitomycin C-arrested mouse embryonic fibroblasts in an undifferentiated state 21 in DMEM/F12 (Gibco by Life Technologies, Paisley, Scotland, UK) medium supplemented with 1% non-essential amino acids, 20% knockout serum replacement, 4 ng/ml bFGF (all from Sigma-Aldrich Chemie GmbH, Steinheim, Germany), and 1 mM L-glutamine (Sigma-Aldrich, St
Enhanced Fat Processing The blend of amino acids optimize liver function and fat breakdown
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