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Conversely, investigations into the mechanism by which G1 mediates body weight and energy balance have revealed an increase in the expression of the thermogenic gene UCP1 [31]

The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed

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This suggests that eating foods high in sulfur and sulfur-containing amino acids could also help glutathione production, as a lack of cysteine is thought to be its main bottleneck [41]

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