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A cellular mechanism to detect and alleviate reductive stress

A cross-linking step [2 mM 3,3-Dithiodipropionic acid di(N-hydroxysuccinimide ester) (DSP, Sigma-Aldrich) for 30 minutes at room temperature followed by quenching with 20 mM Tris HCl for 15 minutes and 3 PBS washes] was performed prior to cell lysis for the SPHKAP-EGFP co-immunoprecipitation experiments to preserve protein-protein interactions despite proteolytic cleavage of the full-length fusion protein during the IP process

While not a prescription medication, gut-directed hypnotherapy and other behavioral therapies are increasingly recognized and often recommended by GI specialists as an adjunctive approach for persistent functional GI symptoms, including bloating and discomfort

Wrap your case in an additional insulating layer (even a sweatshirt works)

Jul 2022;201:115067