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These innovations not only deepen our understanding of neurotransmitter dynamics and physiological signaling but also hold promise for transformative applications in disease diagnostics, personalized medicine, and neuroengineering

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Animals A total of 90 adult male Wistar rats, weighing approximately 200 to 250 g, were purchased from the Animal Experiment Center of the Chinese Academy of Medical Sciences, Beijing, China and were maintained in wooden cages at a temperature of 27 C30 C, with a 12 h light-dark photo-periodicity cycle