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doi:10.1369/0022155413497586 70
The initiation of cuproptosis critically depends on mitochondrial oxidative stress, as the oxidation of lipoyl groups on target proteins is a prerequisite for their high-affinity binding to Cu, which drives toxic protein aggregation [3]
the MichaelisMenten constant ( K m ), maximal velocity (V max ), turnover number ( k cat ) of TyrBm and the inhibition constants ( K I, K IS ) of each inhibitor were determined by LineweaverBurk plot analysis using various concentrations of L-tyrosine (0.031.4 mM) and L-dopa (0.152.0 mM) as substrates
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