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aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF + aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 "10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 "10 M Dihexa (Harding lab) in aCSF, 10 "12 M Dihexa in aCSF or 50 ng/ml HGF + 10 " ' 2 M Dihexa in aCSF for 30 minutes at 37C with gentle rocking

b Infusion of 4F-PCC

Storage temperature range (28C) and shelf life (28 days refrigerated) are the same, but GHK-Cu requires amber or opaque containers as a non-negotiable addition

10.1126/sciadv.1701679 146 QosaH.LichterJ.SarloM.MarkandaiahS

Product information on every package Each unit is labeled with batch number, expiration date, and concentration ensuring maximum transparency

Another study found that EGCG can reverse homocysteine-induced neurodegeneration and neuroinflammation in hyper-homocysteinemic rats