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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (EM1901-56) at 1/2,000 dilution for 1 hour at room temperature

and integrins such as v3, v5, 51, and 53 have crucial roles in the recruitment, migration, and activation of inflammatory cells

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Step 3: Transfer to intrinsic factor