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After 6 h of simulated ischemia, reperfusion was initiated by buffer exchange to normal culture medium in 95% room air and 5% CO2 for 12 h

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After pre-conditioning in the presence or absence of 50 nM Exendin-4 for additional 18 hours, culture medium was changed to serum-free DMEM containing 1 mM sodium pyruvate, without glucose and sodium bicarbonate

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