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Billings, FTt, Hendricks PA, Schildcrout JS, Shi Y, Petracek MR, et al

Then, the fresh culture medium was added to the cells and incubated for another 20 h

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Metabolomics analysis showed a decrease in NADPH in hypoxic mutant chondrocytes, both during proliferation and differentiation, resulting in a reduced NADPH:NADP + ratio, indicating that hypoxic chondrocytes depend on the PPP for sufficient NADPH production and that other NADPH-generating pathways cannot compensate (Fig