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M9 minimal salt medium containing 5 g/L glucose, 15.1 g/L Na 2 HPO 4 , 3 g/L KH 2 PO 4 , 0.5 g/L NaCl, 1 g/L NH 4 Cl, 0.5 g/L MgSO 4 7H 2 O, 0.01 g/L CaCl 2 , 0.2 mL/L 1 % vitamin B1 and a 0.05 mL/L stock solution of trace elements was used for the cultivation of engineered E

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The first set was homogenized in cold PBS (pH 7.4) using a Teflon homogenizer, and the homogenates were centrifuged at 14,000 g for 20 min at 4 C