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Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks
The lyophilised peptide is carefully measured and packaged to maintain its integrity
Treat 15.7% as an upper-bound estimate for pure complex, and understand that the real figure for any given vial is unknown to the buyer
Preventative health and wellness are at the heart of everything we do
DOI: 10.1093/oxfordjournals.humrep.a136291 45
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