Citation23 In Enterobacteriaceae , a variety of -lactamases, including extended-spectrum -lactamases (ESBLs) and carbapenemases (KPC, NDM, VIM, OXA), are present and confer resistance against last-resort antibiotics like carbapenems
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However, after we added a bacterial-type selenocysteine insertion sequence element to the open reading frame we successfully expressed TGR, using the strategy previously employed for production of mammalian TrxR [14,15]
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