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More than four or five carbons in a monohydroxy alcohol, aldehyde, ketone, or acid cannot penetrate the hydrogen bonding structure of water

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Enzymatic activity of the unheated CAT solution alone was defined as 100%

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The next day, samples were incubated with FITC-conjugated, TRITC-conjugated, or Cy5-conjugated (1:1000, Invitrogen, United States) secondary antibody along with 4,6-diamidino-2-phenylindole (DAPI) (1:1000, Sigma-Aldrich, United States) at room temperature for 1 h