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Mutated GST sequences were subcloned into pET-15b, pET-24d, pGEX 4T-1, and pCMV-2B expression vectors, and the mutated constructs were confirmed by DNA sequencing

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Some studies and experimental data support the existence of mitochondria-independent necroptotic pathways and the ability of cells to undergo necroptosis following mitophagy-mediated depletion of mitochondria [135, 136]

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Cofactors can be considered "helper molecules" that assist in biochemical transformations

Notably, AIM2 confers resistance even in treatment-nave cells, positioning it as both a biomarker and a therapeutic target