This is a chemically manufactured medication that does contain natural amino acids but they are manufactured in a lab into a protein sequence that is not natural. This is NOT like how we give women bioidentical hormones from yams or soy
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Proteins were clarified by centrifugation, quantified by BCA assay, reduced with DTT, alkylated with iodoacetamide, diluted and sequentially digested with LysC (1:50, 2 h) and trypsin (1:50, 14 h)
In our niclosamide solution approach they are not present and so cannot exert any deleterious equilibrating effects, i.e., the high niclosamide solution concentration is preserved
Furthermore, the strategy of multi-copy sod -expressing cassettes was employed to improve SOD activity