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Cryosection Cochleae from P3, P14, and P30 C57BL/6 WT mice were dissected out and fixed with 4% paraformaldehyde (PFA) in PBS at 4C overnight

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However, unless the protein is unfolded to expose all the cysteines, only surface-exposed, not buried, free cysteines will be labeled

A provider should decide whether that discussion fits the patients health history and current concerns

Wright 1999, PMID 10234025

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