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This is where the triple mechanism begins showing meaningful metabolic effects

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As FITC fluorescence is pH-dependent, we analyzed the pH differences in the internalized FITC-agonist environment by measuring their fluorescence in cells treated with or without bafilomycin, a vacuolar-type H + ATPase which restores neutral endosomal pH 30

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10.1056/NEJMc2513088 4 AgarwalR.GreenJ