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To illustrate this unit, see the following video: 1.2 Phase I and Phase II reactions Metabolism requires the contribution of different xenobiotic-metabolizing enzymes, which occur at the main locations of possible chemical exposure, namely the lungs, gastrointestinal track, skin and eyes, being present as well in the kidneys, many endocrine glands, the brain and the red blood cells

This process involves having enough magnesium, zinc, vitamins B1, B2, B6 and B12 and folate, as well as enough methyl groups

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Then, tissues were mechanically homogenized by passage through 70 m cell strainers, and the resulting homogenates were serially diluted in sterile PBS