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The isolated mRNA was reverse transcribed via the Superscript III First-Strand Synthesis System (ThermoFisher Scientific) according to manufacturers instructions

10.12938/bmfh.17-018

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As IKK- is the direct upstream kinase that phosphorylates IB-, leading to its degradation, we next examined whether decreased TNF-induced IB- phosphorylation by H 2 O 2 treatment was due to its effects on IKK- activity