Although there are numerous chromatographic methods for the determination of Cys, GSH and Hcy in biological samples based on spectrophotometric, spectrofluorimetric and mass spectrometry detection 18,19,20 , only two are dedicated for N-Hcy-Lys measurement 17,21
The proteins were embedded in a box of OPC water molecules (minimum distance of 12 from the solute to the boundaries of the box) and the net charge of the system was set to zero by neutralization with Cl-ions using the program tLEaP included in AmberTools21 ( 60
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Singh Y, Meher JG, Raval K, Khan FA, Chaurasia M, Jain NK, et al