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A 12-week randomized clinical trial cited across NIH/PMC-indexed research on GHK-Cu's regenerative actions found significant anti-aging benefits from a GHK-Cu facial cream applied twice daily, including reduced fine lines, coarse wrinkles and mottled pigmentation, increased skin density and thickness, and strongly stimulated keratinocyte proliferation

Human Equivalent Dose Calculations Using standard conversion formulas accounting for body surface area differences between species, a rat oral dose of 0.0045 mg/lb translates to approximately 0.00073 mg/lb in humans, yielding roughly 0.112 mg/day for a 154-pound person