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Expression of mutant OCTN2 cDNAs in mammalian cells To confirm their causative role, the R19P and R399Q mutations were created by site-directed mutagenesis in a mammalian expression vector and transfected into CHO cells (Fig

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88 In terms of immune regulation, it has been documented that interleukin-4 (IL-4) and IL-13 inhibit the expression of GPX4 in some tissues (including heart, spleen, lung, and kidney), while the expression of ALOX15 increases, resulting in the massive production of arachidonic acid metabolites and inducing lipid peroxidation