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Blood Sample Preparation Upon collection into polypropylene rnicrofuge tubes without heparin, blood samples were immediately centrifuged at 4C, 2300x g for 5 min to remove any cells and clots and the serum transferred into clean microcentrifuge tubes

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Poznyak, A

Gu S, et al

Cell Signal 42:281291

ROS, such as superoxide (O 2 ) and hydrogen peroxide (H 2 O 2 ), also serve as signaling molecules at low levels, modulating metabolic and vascular functions (Sies et al., 2020)