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Single-nucleus RNA sequencing analysis Using a previously published single-nucleus RNA sequencing atlas 39 , we calculated the number of Glp1r + cells and other markers of interest and quantified their overlap, defining positive cells as those with mRNA counts greater than 0

doi: 10.3945/ajcn.116.139337 132 AmesBNWakimotoP

Disc height indexes (DHIs) were calculated by three spinal surgeons who were blinded to the treatment groups as previously described with minor modifications (DHI = IVD height/adjacent IVD body height)

Sanitizing Vials and Equipment Alcohol swabs clean rubber stoppers before needle insertion occurs

Recommended daily intake for adults is 1.5 micrograms according to UK dietary reference values