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The rAAV-Ef1-DIO-mCherry-WPRE-pA (AAV-DIO-mCherry, AAV2/9, 2.12 10 12 vg/mL) virus was used as the control

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A green eye can be nudged toward gray or made lighter, but is hard to make blue or brown

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This observation means that xCT blockage induces the accumulation of glutamate that is converted into -ketoglutarate instead of being used in glutamate-cysteine exchange and consequently that is why cysteine does not enter the cell and give rise to glutathione