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Mannervik B, Alin P, Guthenberg C, Jensson H, Tahir MK, Warholm M et al

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Due to the polarity of GSH, thiols, amino acids, and peptides, derivatization is often required for RP separation, extraction, or UV/fluorescence detection, using reagents like N-ethylmaleimide (NEM) [31, 50, 54], 5,5-dithiobis(2-nitrobenzoic acid) (Ellmans reagent) [33, 55], iodoacetic acid [56], orthophthalaldehyde [34, 35], monochlorobimane [49], or monobromobimane [35]

Dunnill C, Patton T, Brennan J, Barrett J, Dryden M, Cooke J, et al