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In the linear regression test (Table 3), beta coefficient ( - 0.61) was obtained, indicating a significant negative effect between the MASI score and plasma GSH levels in the melasma patient

Samples were postfixed with 1% osmium tetroxide in veronal acetate buffer (pH 7.4) for 1 h at 25 C, stained with uranyl acetate (5 mg/ml) for 1 h at 25 C, dehydrated in acetone, and embedded in Epon 812 (EMbed 812, Electron Microscopy Science, Hatfield, PA, USA)