Blood tests may be needed to determine deficiencies and proper dosing
The supernatants were boiled in loading buffer (Sangon Biotech, China) for 10 min
Both of these growth factors have a similar structure to insulin

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"Physiological regulation of the human growth hormone (GH)insulin-like growth factor type I (IGF-I) axis: predominance of pulsatile GH secretion and role of IGF-I feedback." Endocr Rev