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Ford AC, Forman D, Hunt R, et al

So we developed a solution

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Reconstituted Storage With 3.0 mL bacteriostatic water, 28C for up to 28 days

Secondary antibodies were applied for 4 hours, after which sections were washed and placed in FluoroShield medium containing DAPI fluorochrome (4',6-diamidino-2-phenylindole) for nuclear staining