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Cell culture Rat pheochromocytoma cells (PC12) were obtained from the American Type Culture Collection (Manassas, VA, USA), and were cultured in Dulbeccos Modified Eagles medium supplemented with 6% fetal bovine serum (heat inactivated), 6% horse serum (heat inactivated), 1 mm glutamine, 100U ml 1 penicillin and 100 g ml 1 streptomycin at 37 C in a humidified 5% CO 2 incubator