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10.3945/jn.112.172395 J Nutr

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To identify novel brain and circuit targets for addiction intervention, preclinical rodent models are utilized because brain cell types are genetically accessible, and mice are readily compatible with modern systems neuroscience approaches such at two-photon calcium imaging

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Tissues were voltage-clamped at 0 mV using an automatic voltage clamp (EVC 4000, World Precision Instruments, Sarasota, FL, USA) and the short-circuit current (I SC ) required to maintain the 0 mV potential was monitored as a recording of the net active ion transport across the epithelium