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The position of SLC33A1 MAP in a membrane was calculated using the Orientation of Proteins in Membranes (OPM) PPM 2.0 web server 70

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10.1186/1742-2094-8-117 J

This is supported by the observation that basal acidification and glycolytic reserve were at basal levels at 24 h, but the compensatory glycolysis was increased at this time point

Oocyte collection and in vitro maturation For in vitro maturation (IVM), bovine ovaries, collected from a local slaughterhouse, were kept in normal saline and transported to the laboratory within 2 h