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Patients in their late 30s to early 50s who have begun noticing collagen loss but have not yet developed deep structural atrophy represent the sweet spot

For siRNA transfection, 3.6 l of aliquoted siRNA was dispensed to flat-bottom 96-well plates (Nunc MicroWell, 167008)

NADPH-dependent glutathione reductase turns GSSG back into GSH, completing the cycle

Glutathione is a non-toxic antioxidant that the body makes naturally

Schielke et al., 2010), which, as mentioned above, was implicated in diverse functions including adherence to epithelial cells, intracellular survival, vacuolar escape, interaction with dynein, and modulation of apoptosis/pyroptosis (Schmitt et al., 2007

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