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doi: 10.3389/fendo.2013.00076 6 SpitzCWinkelsHBrgerCWeberCLutgensEHanssonGKet al
whether the washing solution provided in the kit is used 3.The washing liquid is contaminatedPrepare new lotion Problem description: Low sensitivityPossible causes and corresponding countermeasures: 1.ELISAImproper storage of the test kitStore relevant reagents according to the instructions 2.Not terminated before readingODStop solution should be added to each well before reading
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The antioxidant activity of melanoidins isolated from different brews was studied, and some showed that the radical scavenging ability of coffee melanoidins was higher in the dark-roasted coffee, others exhibited higher radical scavenging activity, and still others showed radical scavenging ability of coffee melanoidins was not affected by the degree of roasting (Tagliazucchi, 2010)