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Staining for annexin V and Hoechst 33342 RINm5F cells were cultured in a 24-well plate

The product was diluted to a volume of 30 l, and aliquots of 1 l were then analysed using individual probes (final concentration = 1) for a further 45 cycles using 7900 HT Fast Real-Time PCR system (Applied Biosystems)

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Given its biological effects, activation of GLP-1R has been explored as a latent treatment for metabolic disorders like type 2 diabetes mellitus (T2DM) and obesity

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