Samples were run on Tris-tricine or Tris-HCl SDS-PAGE gels and transferred to 0.45 m Immobilon-P Polyvinylidene Fluoride (PVDF) (Millipore) for Western blotting using ECL Prime substrate (GE Healthcare)
The biochemical analysis of the groups involved measuring the amounts of IL-1 and TNF- in the supernatants using ELISA kits specifically designed for rats (Cat
Liu J, Huang Y, Kumar A, Tan A, Jin S, Mozhi A, et al
PMID: 14 TangDKroemerGKangR
In conclusion, OA pathogenesis is propelled by a metabolically dysregulated milieu, where obesity, oxidative stress, gut dysbiosis, and broader metabolic syndrome converge to create a self-perpetuating cycle of inflammation and tissue damage (Figure 1)
It features a powerful mix of L-Glutathione, Vitamin C, and Alpha Lipoic Acid