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Further, although several previous studies have evaluated GSH levels in various extracellular compartments, including bronchoalveolar lavage (BAL) fluid, these studies generally focused on alterations in GSH:GSSG balance in association with disease, or the consumption of GSH during prostaglandin or leukotriene synthesis, with more modest phenotypic, pathophysiologic, or mechanistic information (4, 5)

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HT and NAC combination assay After being inoculated, the cells were cultured for 24 h and then treated with NAC at various concentrations (0, 1, 2, 4, and 8 mM) for 48 h, after which the change in the OD value at 450 nm was determined