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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

This medical support ensures patients stay on track, manage side effects effectively, and achieve the best possible long-term results

For broader treatment placement in type 2 diabetes, see Injectable Type 2 Diabetes for context on when GLP-1 RAs complement other classes

While metformin remains the foundational first-line therapy for type 2 diabetes, the 2026 ADA guidelines strongly advocate for a cardiovascular, kidney, and metabolic (CKM) framework , promoting the earlier use of agents with proven organ-protective benefits

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Insulin directly lowers blood sugar by facilitating its absorption into tissues