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British journal of urology
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In contrast to the enhancement of spontaneous excitatory postsynaptic transmission (Mietlicki-Baase et al., 2013), using retrograde labeling of VTA to nucleus accumbens (NAc) medial shell projecting neurons, in vitro patch-clamp recordings showed that exendin-4 selectively inhibits the miniature excitatory postsynaptic currents (mEPSCs) within the dopaminergic VTA-to-NAc projection neurons (Wang et al., 2015
C2C12 cells were differentiated into myotubes by growing them in DMEM for 24 h and then changing the media to DMEM supplemented with 2% horse serum,1 mM sodium pyruvate, and 10 mM HEPES for 3 days [26, 27]
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