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it is prepared per patient prescription by a US-licensed compounding pharmacy

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For the internalization, HEK293A cells (15,000 cells/well) were seeded in a white 384-well plate and transiently transfected with SNAP-hGLP-1R (2.5 ng/well) using Lipofectamine 2000 + Opti-MEM in supplemented DMEM, followed by incubated at 37 C

They note that targeting a normal BMI of under 25 kg/m 2 is neither realistic nor appropriate for many patients living with obesity

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