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In the liver, the 25-hydroxylase enzymes (CYP2R1 and CYP27A1) convert inactive cholecalciferol to 25(OH)VD 3 (calcidiol) by carbon 25 hydroxylation 14

Combining them raises the theoretical risk of additive or even synergistic hepatocellular stress through at least two overlapping mechanisms

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Psychopharmacology 232(3):501507 Guan M, Liu X, Guo L et al (2021) Improved Pre-attentive Processing With Occipital rTMS Treatment in Major Depressive Disorder Patients Revealed by MMN

For formulations requiring both vitamin C and GHK-Cu, apply them in separate steps with a 1015 minute interval, using the vitamin C serum first followed by thorough absorption before applying the copper peptide layer

10.1164/ajrccm.162.1.9908105