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doi: 10.3748/wjg.v24.i29.3204 [DOI] [PMC free article] [PubMed] [Google Scholar] Gray B

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cDNA was synthesized using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen, Grand Island, NY), according to the manufacturer's guidelines

Due to the relatively rapid hydrolysis rate of the imine linker between PEG and T ECM -NS, about 85% of PEG was removed from PEG-T ECM -NS within 2 h