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(2017) PLoS ONE | van der Veen J
Membranes were resuspended in 2xTBS pH 8.0, 2% DDM, 0.2% CHS to a total volume of 100 mL and solubilised with gentle stirring for 1 h at 4 C
About a quarter of women will have RLS while expecting, typically in the second or third trimester
Additionally, vegetarians, particularly vegans, are at a higher risk for Se deficiency, because plant foods might contain low levels of Se, while in some countries, animal feed is fortified with Se (56, 57, 69)
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