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Amino acids V12 (putative oxyanion hole), F112 (active site architecture), N108 (flavonoid binding) and C13 (GSH stabilization) were fully randomized by site saturation mutagenesis (NNK codons), and the created Pt GSTF8 variants were tested for conversion of flavan-3,3,4-triol ( 4 ) to cyanidin ( 8 ), and for their GSH-transferase activity towards CDNB ( 5 ) as a control of catalytic competence
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Materials and methods Animals and experimental procedures For the in vivo analysis, six male newborn Wistar rats (postnatal days 511) were used
We have used a HILIC column to successfully separate polar and ionic/ionizable analytes which include various excipients, the API, and related impurities before detection using an evaporative light scattering detector (ELSD) and MS
doi: 10.1001/archinte.167.11.1145