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The cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (12 10 5 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene
Angelman syndrome (AS) is a rare genetic neurodevelopmental disorder occurring in ~1:15,000 live births, which is characterized by a developmental delay, lack of speech, motor dysfunction, epilepsy, happy demeanor, and intellectual disability [2]
10.1073/pnas.1419737111 16 HornbeckP
Normalized relative abundances of identified peaks were used to calculate fold changes of metabolites among the treatments
4 Borde-ChicheP.DiederichM.MorceauF.PugaA.WellmanM.DicatoM
Acetaldehyde, a byproduct of alcohol, disrupts the conversion of homocysteine to methionine, lowering cysteine and glutathione production and increasing homocysteine levels
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