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The lysate was filtered with a 0.45 m filter and loaded onto a Ni-NTA (Carl Roth, Germany) gravity column equilibrated with buffer A (20 mM sodium phosphate pH 7.5, 500 mM NaCl and 12.5 mM imidazole)

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Conversely, with conditions leading to neurodegeneration or chronic inflammation, oxidative stress persists long enough to lead to abnormal immune response pathwaysparticularly NF-kband prolonged treatment with GSH may lead to reduced redox tone and thus attenuating NF-kB, causing an anti-inflammatory response instead of an immuno-supportive one (Limongi et al., 2019

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