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After incubating with 5% bovine serum albumin for 1 h, the membranes were incubated with primary antibodies overnight at 4 C

Cheng, Jin-Pei

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GHRH Receptor Activation and cAMP Signalling In canonical models, GHRH receptor engagement is associated with adenylate cyclase activation and increased intracellular cAMP, supporting downstream phosphorylation events and regulated gene-expression programs relevant to somatotroph and endocrine-axis function