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Quantitatively, the presence of pyocyanin significantly increased the total biovolume, biofilm thickness, and biofilm surface coverage of AES-1M biofilms, relative to the control (Fig

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The sections were immersed twice in xylene and absolute ethanol for 10 minutes each, then in 95%, 85%, and 75% ethanol for 5 minutes each, followed by a 15-minute PBS rinse