As well, NADPH is involved in the conversion of oxidized glutathione to glutathione, a process that contributes to cellular antioxidant defenses
Sendamangalam V
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Stoichiometric Equation of the PPP The stoichiometry for its most essential mode: The reactants are glucose 6-phosphate + 2 NADP+ + H2O The products are ribulose 5-phosphate + 2 NADPH + H2 + CO2 Glucose 6-phoshate and Glycolysis Glucose 6-phosphate is the first intermediary product in glycolysis
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Cell pellets were lysed with 800 l of buffer C (20 mM Tris-Cl (pH = 8.0), 150 mM NaCl, 1.5 mM MgCl2, 10 mM KCl, 10% Glycerol, 0.5 mM EDTA, 0.5 % NP-40, 1x protease and 1x phosphatase inhibitors)